Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Ultrasound-Assisted Extraction: Effect of Extraction Time and Solvent Power on the Levels of Polyphenols and Antioxidant Activity of Mesembryanthemum edule L. Aizoaceae Shoots

Hanen Falleh^1,2 , Riadh Ksouri¹, Marrie Elizabeth Lucchessi², Chedly Abdelly¹, Christian Magné²

¹Laboratoire des Plantes extrÃªmophiles, Centre de Biotechnologie de Borj-CÃ©dria, BP 901, 2050 Hammam-lif, Tunisie; ²Laboratoire dâ€™Ecophysiologie et de Biotechnologies des Halophytes et des Algues Marines, Institut Universitaire EuropÃ©en de la Mer, TechnopÃ´le de Brest Iroise, 29280 PlouzanÃ©, France.

For correspondence:- Hanen Falleh Email: Hanenfalleh@gmail.com Tel:+21679412848

Received: 16 November 2010 Accepted: 6 February 2012 Published: 24 April 2012

Citation: Falleh H, Ksouri R, Lucchessi ME, Abdelly C, Magné C. Ultrasound-Assisted Extraction: Effect of Extraction Time and Solvent Power on the Levels of Polyphenols and Antioxidant Activity of Mesembryanthemum edule L. Aizoaceae Shoots. Trop J Pharm Res 2012; 11(2):243-249 doi: 10.4314/tjpr.v11i2.10

© 2012 The authors.
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Abstract

Purpose: To investigate the influence of extraction conditions assisted by ultrasound on the quality of extracts obtained from Mesembryanthemum edule shoots.

Methods: The extraction procedure was carried out in an ultrasonic bath. The effect of two solvents (methanol and ethanol) and two extraction times (5 and 10 min) were evaluated on the basis of the phenolic content and antioxidant activity of the plant extract.

Results: Significant variability in phenolic content and antioxidant activity, depending on the solvent (S), and to a lesser degree, extraction duration (D) and their interaction (S/D) were found. Methanol extract was significantly richer in total polyphenols than ethanol extract, their levels being 104.7 and 74.2 mg GAE.g^-1DW, respectively. For the two solvents used, longer extraction duration yielded higher polyphenol content. The ethanol extract exhibited higher antioxidant activity than the methanol extract. Moreover, Also, the longer sonication duration (10 min) yielded extracts with higher antioxidant activities.

Conclusion: The antioxidant capacity of M. edule is strongly influenced by the nature of the extracting solvent and the duration of sonication extraction.

Keywords: Antioxidant activity, Extraction duration, Mesembryanthemum edule, Polyphenols, Sonication